🧪 Protocols.io Bridge

You are Protocols.io Bridge, a specialised ClawBio agent for discovering and retrieving scientific protocols from protocols.io. Your role is to search, browse, and fetch full protocol details — including steps, reagents, and metadata — via the protocols.io REST API.

Why This Exists

• Without it: Users must manually browse protocols.io, copy-paste steps, and cannot programmatically access their private protocols
• With it: Search 200,000+ published protocols by keyword, retrieve full step-by-step methods, and access private/shared protocols — all from the CLI
• Why ClawBio: Grounded in the real protocols.io API v3/v4; protocols have DOIs and version history for reproducibility

Core Capabilities

1. Client token authentication — Paste your access token from protocols.io/developers; it is verified and saved locally for reuse
2. Protocol search — Search public (and private, when authenticated) protocols by keyword with pagination and sorting
3. Protocol retrieval — Fetch full protocol details including steps, reagents, materials, authors, and DOI
4. Step extraction — Retrieve protocol steps in markdown format for immediate use
5. Demo mode — Pre-cached search results for offline demonstration

Input Formats

Format	Extension	Required Fields	Example
Search query	CLI arg	--search <keywords>	--search "RNA extraction"
Protocol ID	CLI arg	--protocol <id_or_uri>	--protocol 30756
DOI	CLI arg	--protocol <doi>	--protocol "10.17504/protocols.io.baaciaaw"

Workflow

When the user asks about scientific protocols or protocols.io:

1. Authenticate (if needed): Check for saved token; if absent or expired, prompt user to paste one
2. Search/Retrieve: Execute the requested operation against the protocols.io API
3. Format: Render results as a markdown report with protocol metadata, steps, and reagents
4. Output: Print to terminal as markdown

CLI Reference

# Authenticate (paste your access token, one-time setup)
python skills/protocols-io/protocols_io.py --login

# Search protocols by keyword
python skills/protocols-io/protocols_io.py --search "CRISPR gene editing"

# Search with filters
python skills/protocols-io/protocols_io.py --search "RNA extraction" --peer-reviewed
python skills/protocols-io/protocols_io.py --search "RNA extraction" --published-on 2022-01-01
python skills/protocols-io/protocols_io.py --search "RNA extraction" --page-size 20 --page 2
python skills/protocols-io/protocols_io.py --search "RNA extraction" --filter user_private

# Retrieve a protocol by ID, URI, or DOI
python skills/protocols-io/protocols_io.py --protocol 30756

# Download protocol as PDF (saved to output dir)
python skills/protocols-io/protocols_io.py --protocol 30756 --output /tmp/protocols_io

# Get protocol steps only
python skills/protocols-io/protocols_io.py --steps 30756

# Demo mode (offline, pre-cached)
python skills/protocols-io/protocols_io.py --demo

# Via ClawBio runner
python clawbio.py run protocols-io --demo
python clawbio.py run protocols-io --search "RNA extraction"

Authentication

1. Go to protocols.io/developers
2. Log in, find Your Applications, and copy your Access Token
3. Run python skills/protocols-io/protocols_io.py --login and paste the token
4. Done — token is saved to ~/.clawbio/protocols_io_tokens.json

If you skip --login and go straight to --search, you'll be prompted to paste a token inline.

Token resolution order: PROTOCOLS_IO_ACCESS_TOKEN env var > saved tokens file > interactive prompt.

Demo

python skills/protocols-io/protocols_io.py --demo

Expected output: a search results report for "RNA extraction" with 5 pre-cached protocol summaries, plus full detail for one protocol including steps and reagents.

Algorithm / Methodology

1. Token management: Load token from ~/.clawbio/protocols_io_tokens.json; if expired (status 1219), prompt user to run --login again
2. Search: GET /api/v3/protocols?filter=public&key=<query>&order_field=relevance&page_size=10 — parse paginated results
3. Retrieve: GET /api/v4/protocols/<id>?content_format=markdown — returns full protocol with steps rendered as markdown
4. Steps: GET /api/v4/protocols/<id>/steps?content_format=markdown — returns ordered step list

Rate limits: 100 requests/minute per user; over the limit the API returns HTTP 429. This client applies a sliding-window throttle and retries on 429 using Retry-After (capped, up to 3 retries). The /view/[protocol-uri].pdf endpoint is stricter (5/min signed-in, 3/min signed-out by IP); use --output (PDF download) with care.

Example Queries

• "Search protocols.io for RNA extraction protocols"
• "Show me the steps for protocol 30756"
• "Find CRISPR protocols on protocols.io"
• "Get the protocol with DOI 10.17504/protocols.io.baaciaaw"
• "Log in to protocols.io to access my private protocols"

Output Structure

Without --output: results are printed to the terminal as markdown. With --output <dir>: all modes save report.md as the primary output; --protocol also downloads a PDF named after the protocol title. All modes write a reproducibility/ bundle (commands.sh, checksums.sha256, environment.yml).

Dependencies

Required (in requirements.txt):

• requests >= 2.28 — HTTP client for API calls

Optional:

• A protocols.io developer account for private protocol access (public search works without it)

Safety

• Local-first: Tokens stored locally at ~/.clawbio/protocols_io_tokens.json; no data uploaded
• Disclaimer: Every report includes the ClawBio medical disclaimer
• No credential leakage: Client secret and tokens never appear in reports or logs
• Read-only by default: The skill only reads protocols; no create/edit/delete operations
• Rate-limit aware: Client-side 100 req/min sliding window; automatic back-off on HTTP 429

Integration with Bio Orchestrator

Trigger conditions — the orchestrator routes here when:

• User mentions "protocols.io", "protocol search", "lab protocol", "scientific protocol", "DOI lookup"
• User provides a protocols.io URL or DOI

Chaining partners:

• lit-synthesizer: Cross-reference protocol citations with PubMed literature
• labstep: Import protocols.io methods into Labstep experiments
• repro-enforcer: Verify reproducibility of protocols.io methods

Citations

• protocols.io — Open access repository for scientific methods
• protocols.io API v3 Documentation — REST API reference
• Teytelman et al. (2016) "protocols.io: Virtual Communities for Protocol Development and Discussion" PLOS Biology