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Cultivate edible and medicinal mushrooms from spawn through fruiting at home scale.
Match species to your environment and experience level.
Beginner-Friendly Species:
+--------------------+------------------+------------------+------------------+
| Species | Substrate | Temperature | Difficulty |
+--------------------+------------------+------------------+------------------+
| Oyster mushroom | Straw, coffee | 15-24C (60-75F) | Very easy |
| (Pleurotus spp.) | grounds, sawdust | | (most forgiving) |
+--------------------+------------------+------------------+------------------+
| Shiitake | Hardwood logs | 13-21C (55-70F) | Easy |
| (Lentinula edodes) | or sawdust blocks| | (outdoor logs) |
+--------------------+------------------+------------------+------------------+
| Lion's mane | Hardwood sawdust | 18-24C (65-75F) | Moderate |
| (Hericium | (supplemented) | | (needs humidity) |
| erinaceus) | | | |
+--------------------+------------------+------------------+------------------+
| Wine cap | Wood chips, | 10-27C (50-80F) | Easy |
| (Stropharia | straw mulch | | (outdoor beds) |
| rugosoannulata) | (outdoor beds) | | |
+--------------------+------------------+------------------+------------------+
Start with oyster mushrooms — they colonize fast, fruit reliably,
and tolerate imperfect conditions.
Expected: Species selected that matches your environment, substrate availability, and experience level.
On failure: If unsure, start with blue oyster mushroom (Pleurotus ostreatus) on straw. It is the most forgiving species for beginners.
The substrate provides nutrition for the mycelium. It must be clean enough to give your mushroom a head start over competitors.
Substrate Preparation Methods:
PASTEURIZATION (for straw — easiest):
1. Chop straw to 2-4 inch lengths
2. Submerge in hot water (65-80C / 150-175F) for 60-90 minutes
3. Drain thoroughly — substrate should be moist but not dripping
(squeeze test: a firm squeeze produces a few drops, not a stream)
4. Cool to below 30C (85F) before inoculation
STERILIZATION (for supplemented sawdust — more reliable):
1. Mix hardwood sawdust with 10-20% wheat bran or soy hull
2. Hydrate to 60-65% moisture content
3. Fill into autoclavable bags with filter patches
4. Pressure cook at 15 PSI for 90-120 minutes
5. Cool completely before inoculation (overnight is safest)
COLD WATER LIME BATH (alternative pasteurization):
1. Dissolve hydrated lime (calcium hydroxide) in cold water
(approximately 1 cup per 50 gallons)
2. pH should reach 12+ (kills competitors without heat)
3. Soak straw for 12-18 hours
4. Drain and let excess water drip for 2-4 hours
5. pH will neutralize as the straw dries
Expected: Substrate is clean (pasteurized or sterilized), at correct moisture content, and cooled to room temperature.
On failure: If contamination appears after inoculation (green mold within the first week), the substrate was insufficiently pasteurized or the inoculation environment was too dirty. Start fresh with more rigorous pasteurization.
Introduce spawn to the prepared substrate.
Inoculation Protocol:
1. Work in a clean environment: wash hands, clean surfaces, minimize airflow
(still air is better than a breeze carrying contaminants)
2. Spawn rate: 10-20% spawn by weight relative to wet substrate
(more spawn = faster colonization = less contamination risk)
3. Mix spawn thoroughly into the substrate (for bags/buckets)
OR layer spawn between substrate layers
4. Pack into growing container:
- Grow bags: fill loosely, fold and clip top
- 5-gallon buckets: drill 1/2" holes in sides (every 6 inches),
fill with inoculated substrate, cap loosely
- Logs: drill holes, insert plug spawn, seal with wax
5. Label with species, date, and substrate type
Hygiene Priorities:
- Clean hands and surfaces
- Minimize time substrate is exposed to open air
- Work quickly and confidently
- If you touch a contaminated surface, re-clean before continuing
Expected: Spawn is evenly distributed throughout the substrate in a clean container, ready for incubation.
On failure: If spawn does not seem to be colonizing after 7-10 days (no visible white growth), check temperature (too cold slows growth), substrate moisture (too dry inhibits growth), and spawn viability (old or heat-damaged spawn may be dead).
The mycelium colonizes the substrate during incubation.
Incubation Conditions:
+--------------------+------------------------------------------+
| Parameter | Target |
+--------------------+------------------------------------------+
| Temperature | Species-specific (generally 20-25C / |
| | 68-77F for most species) |
+--------------------+------------------------------------------+
| Light | Dark or dim — direct light not needed |
| | during colonization |
+--------------------+------------------------------------------+
| Air exchange | Minimal — CO2 buildup is acceptable |
| | during colonization (loose lid is enough)|
+--------------------+------------------------------------------+
| Duration | 2-4 weeks (until substrate is fully |
| | white with mycelium) |
+--------------------+------------------------------------------+
| Monitoring | Check every 3-4 days for contamination |
| | (green, black, orange, or pink mold) |
+--------------------+------------------------------------------+
Contamination Response:
- Green mold (Trichoderma): most common competitor. If localized and
small, remove the contaminated area. If widespread, discard the
entire block/bag — Trichoderma wins once established.
- Black mold: discard immediately. Do not open indoors.
- Orange/pink: bacterial contamination from wet substrate. Discard.
Expected: Full colonization — the substrate is uniformly white with mycelium and smells pleasantly mushroomy.
On failure: Partial colonization with contamination means the race was lost. Start again with more spawn (higher ratio), better pasteurization, and cleaner inoculation practice.
Trigger the transition from vegetative growth to mushroom formation.
Fruiting Triggers:
1. Fresh air: increase air exchange (open container, fan nearby)
2. Light: indirect light for 12 hours/day (any spectrum works)
3. Temperature drop: reduce by 5-10C from incubation temperature
4. Humidity: maintain 85-95% relative humidity
- Mist 2-3 times daily
- Or use a fruiting chamber (plastic tub with perlite floor)
5. For bags: cut X-shaped slits where you want mushrooms to emerge
For buckets: mushrooms emerge from the drilled holes
Fruiting Chamber (Simple SGFC — Shotgun Fruiting Chamber):
- Large plastic storage tub (50-100L)
- Drill 1/4" holes every 2 inches on all 6 sides (including bottom and lid)
- 4-5 inch layer of wet perlite on the bottom
- Place colonized blocks/bags on a wire rack above the perlite
- Mist walls 2-3 times daily
- Fan fresh air in by waving the lid 2-3 times daily
Expected: Primordia (tiny mushroom pins) appear within 5-14 days of fruiting initiation.
On failure: If no pins appear after 2 weeks: check humidity (too dry is the most common cause), light (some species need light to pin), and temperature (too warm delays pinning for many species).
Harvest Timing:
- Harvest just before or as the cap edges begin to flatten or turn upward
- For oysters: when the cap edges are still slightly curled downward
- For shiitake: when the cap is 70-80% open (partial veil still intact)
- For lion's mane: when spines are 0.5-1 cm long and still firm
Harvest Technique:
- Twist and pull gently at the base (preferred for most species)
- Or cut with a clean knife at the substrate surface
- Do not leave stumps that can rot and attract contamination
Successive Flushes:
- After harvesting, soak the block/bag in cold water for 12-24 hours
(rehydration triggers the next flush)
- Return to fruiting conditions
- Expect 2-4 flushes, each smaller than the last
- Total yield: approximately 25-50% of wet substrate weight
for oyster mushrooms over all flushes
Expected: Fresh mushrooms harvested at optimal timing, with successive flushes extending the productive life of the substrate.
On failure: If yields are poor (small, sparse mushrooms), the substrate may be depleted or contaminated. Supplemented substrates produce higher yields. If contamination appears between flushes, the block's productive life is over — compost it.
fungi-identification — complementary skill; cultivation eliminates identification risk but understanding morphology aids in recognizing contamination speciesprepare-soil — spent mushroom substrate is excellent garden amendment; the cultivation cycle connects to soil building